Protective action of NADPH oxidase inhibitors and role of NADPH oxidase in pathogenesis of colon inflammation in mice

Figure 3 Assessment of viability of mouse colonic epithelial cells. Cells were incubated with 20 μg/mL LPS, 1 mmol/L apocynin (Apoc), 20 μg/mL LPS + 1 mmol/L Apoc, and without stimulation for 24 h. A: Untreated mice; B: Acute DSS-induced colitis group; C: Chronic DSS-induced colitis group. ^aP < 0.05, DSS-induced colitis groups untreated vs one of the stimulated subgroups in the control; ^cP < 0.05, DSS-induced colitis groups vs LPS and LPS + Apoc treated subgroups in the control; ^eP < 0.05, chronic DSS-induced colitis groups in the untreated subgroup vs control; ^gP < 0.05, chronic DSS-induced colitis groups in the LPS subgroup vs control; Values are presented as mean ± SE. Untreated mouse group: n = 12. Both DSS-induced colitis groups: n = 8. LPS: Lipopolysaccharide.