TLR4 signaling and the inhibition of liver hepcidin expression by alcohol

Figure 8 Effect of alcohol on small heterodimer partner and nuclear factor-κB interaction. A: Whole cell lysates isolated from the livers of C3H/HeJ and C3H/HeOuJ mice pair-fed with regular and ethanol-containing diets were immunoprecipitated with an anti-small heterodimer partner (SHP) antibody or anti-rabbit IgG, as control. The expression of nuclear factor-κB in the immune complexes was determined by western blotting using an anti-p65 antibody, as described in Methods; B: p65 protein expression in SHP immune complexes was quantified by densitometric analysis and normalized to control IgG immune complexes. Normalized p65 expression in ethanol-fed mice was expressed as fold expression of that in the respective mice pair-fed with the regular diet (mean; n = 3 per group).