TLR4 signaling and the inhibition of liver hepcidin expression by alcohol

Figure 7 Small heterodimer partner protein expression. Small heterodimer partner (SHP) protein expression was detected by western blots using cytosolic (A, B) and nuclear (C, D) fractions isolated from the livers of C3H/HeJ and C3H/HeOuJ mice pair-fed with regular and ethanol-containing diets. An anti-gapdh antibody (B) was used as a cytosolic protein loading control; D: Nuclear proteins on PVDF blotting membranes were visualized by Ponceau S staining to evaluate equal protein loading. Cytosolic (E) and nuclear (F) SHP expression was quantified by densitometric analysis and normalized to control protein expression. Normalized SHP expression in ethanol-fed mice was expressed as fold expression of that in the respective mice pair-fed with the regular diet (mean; n = 3 per group).