TLR4 signaling and the inhibition of liver hepcidin expression by alcohol

Figure 5 Nuclear factor-κB binding to hepcidin gene promoter. Chromatin isolated from the livers of control or ethanol-fed C3H/HeJ and C3H/HeOuJ mice was immunoprecipitated with an anti-p50 nuclear factor-κB antibody (A) or control IgG (C). Immunoprecipitated DNAs (A, C) and input DNA control (B) were subjected to polymerase chain reaction, as described in Methods.