TLR4 signaling and the inhibition of liver hepcidin expression by alcohol

Figure 4 IκBα expression and SAA3 expression. A: Cytosolic protein fractions isolated from the livers of C3H/HeOuJ and C3H/HeJ mice pair-fed with regular and ethanol-containing diets were used for western blots to detect IκBα expression. An anti-gapdh antibody was used as control; B: IκBα expression was quantified by densitometric analysis and normalized to gapdh expression. Normalized IκBα expression in ethanol-fed mice was expressed as fold expression of that in the respective mice pair-fed with the regular diet (mean; n = 3 per group); C: cDNA, synthesized from RNA isolated from the livers of these mice, was employed in real-time polymerase chain reaction to measure SAA3 mRNA expression. SAA3 expression in ethanol-fed mice was expressed as fold expression of that in the respective mice pair-fed with the regular diet (mean ± SE; n = 10 per group).