TLR4 signaling and the inhibition of liver hepcidin expression by alcohol

Figure 2 Tumor necrosis factor α expression. The level of tumor necrosis factor (TNF)α in sera of toll-like receptor 4 mutant (C3H/HeJ) and wildtype control (C3H/HeOuJ) mice, pair-fed with regular and ethanol-containing diets was determined by ELISA assays, using a commercial kit (BioLegend), according to the manufacturer’s instructions, and expressed as picogram cytokine per ml of serum (mean ± SE; n = 10 per group).