Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester

Figure 2 Protein profiles of SW480 cells treated with caffeic acid phenethyl ester by two-dimensional PAGE. SW480 cells were treated without (A) or with (B) 10 μg/mL caffeic acid phenethyl ester (CAPE) for 48 h and then harvested for 2D-PAGE analysis. 500 mg of cellular protein was applied to the IEF gel. After electrophoresis, the gels were stained with Coomassie brilliant blue R-250 and the proteins were analysed by PDQuest 2-D analysis software. The marked spots shown on the gels were the down-regulated proteins (shown by numbers 1-7) and the up-regulated proteins (shown by numbers 8-11). The relative abundance of each differentially expressed protein in the 2 gels was analysed by PDQuest (C). The first bar shown in the graph is the relative abundance of the proteins in untreated cells and the second bar is the relative abundance of the proteins in CAPE-treated cells. Results are representative of 3 independent experiments with similar results.