Connexin 32 and 43 promoter methylation in Helicobacter pylori-associated gastric tumorigenesis

Figure 3 Methylation-specific polymerase chain reaction results for Cx32 and Cx43 promoters at different gastric carcinogenesis stages with Helicobacter pylori infection. A: The agarose gel electrophoresis of the methylation-specific polymerase chain reaction (MSP) bands. Marker: 50bp ladder; M: Methylated; U: Unmethylated; MP: Methylation positive control; UP: Unmethylated positive control; DW: Negative control. B: The methylation levels of Cx32 and Cx43 promoters at different stages by MSP method. ^aP < 0.05 vs NGM; ^cP < 0.05 vs NAG; ^eP < 0.05 vs CAG; ^gP < 0.05 vs IM; ⁱP < 0.05 vs DYS. NGM: Normal gastric mucosa; NAG: Non-atrophic gastritis; CAG: Chronic atrophic gastritis; IM: Intestinal metaplasia; DYS: Dysplasia; GC: Gastric cancer.