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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Sep 7, 2014; 20(33): 11780-11787
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11780
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11780
Table 1 Immunohistochemistry protocols used to characterize the proteins expression
| Code | Industry | Dilution | Epitope retrieval | Amplification system |
| MCT1 (sc-365501) | Santa Cruz Biotechnology, Santa Cruz, CA, United States | 1:500 | EDTA (1 mmol/L, pH = 8); | Vectastain® Elite ABC reagent |
| 98 °C; 20 min | (Burlingame, CA, United States) | |||
| MCT2 (sc-14926) | Santa Cruz Biotechnology, Santa Cruz, CA, United States | 1:200 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 20 min | Ultravision Detection System Anti-polyvalent, HRP, Lab Vision Corporation, Fremont, CA, United States) |
| MCT4 (sc-50329) | Santa Cruz Biotechnology, Santa Cruz, CA, United States | 1:500 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 20 min | Ultravision Detection System Anti-polyvalent, HRP, Lab Vision Corporation, Fremont, CA, United States) |
| CD147 (sc-71038) | Santa Cruz Biotechnology, Santa Cruz, CA, United States | 1:400 | EDTA (1 mmol/L, pH = 8); | Vectastain® Elite ABC reagent (Burlingame, CA, United States) |
| 98 °C; 20 min | ||||
| GLUT1 (ab15309) | Abcam, Cambridge, United Kingdom | 1:500 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 20 min | Ultravision Detection System Anti-polyvalent, HRP, Lab Vision Corporation, Fremont, CA, United States) |
| Caspase 3 | Diagnostic BioSystems (DBS) Pleasanton, CA, United States | 1:160 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 40 min | NovoLink (Novocastra Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) |
| (3C SP03) | ||||
| Keratin 19 (K19, b170) | Novocastra Laboratories Ltd, Newcastle Upon Tyne, United Kingdom | 1:300 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 40 min | NovoLink (Novocastra Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) |
| Ki-67 (MIB-1) | (Dako, Glostrup, Denmark) | 1:400 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 40 min | NovoLink (Novocastra Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) |
| EGFR human (DAK-H1-WT) | (Dako, Glostrup, Denmark) | 1:200 | Citrate buffer (10 mmol/L, pH = 6); 98 °C; 40 min | NovoLink (Novocastra Laboratories Ltd, Newcastle Upon Tyne, United Kingdom) |
- Citation: Alves VA, Pinheiro C, Morais-Santos F, Felipe-Silva A, Longatto-Filho A, Baltazar F. Characterization of monocarboxylate transporter activity in hepatocellular carcinoma. World J Gastroenterol 2014; 20(33): 11780-11787
- URL: https://www.wjgnet.com/1007-9327/full/v20/i33/11780.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i33.11780