MicroRNA-218 is upregulated in gastric cancer after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy and increases chemosensitivity to cisplatin

Figure 4 miR-218 increased chemosensitivity to cisplatin could be accelerated by overexpression of mTOR. Cells were transfected with miR-NC, or miR-218 with or without mTOR cDNA. Cells were exposed to cisplatin for further detection 10 h after transfection. A: Tumor cells proliferation assay of different cisplatin concentrations. 6 × 10³ cells were seeded in 96-well plates and incubated with different concentration of cisplatin for 72 h. Data are presented as mean ± SD from three independent experiments performed in sextuple; B: Cell proliferation in the presence of 12 μmol/L cisplatin. Data are presented as mean ± SD from three independent experiments performed in sextuple. ^aP < 0.05 vs control; ^cP < 0.05 vs miR-218 + mTOR.