Role of moxibustion in inflammatory responses during treatment of rat ulcerative colitis

Figure 5 Expression of Toll-like receptor 9 and nuclear factor-κB p65 in colonic tissue of rats. A-E corresponds to the expression in colonic tissue of rats from blank control, waiting list control, 3-min, 6-min and 9-min moxibustion groups, respectively. Extracts of colonic tissues (approximately 25 μg) from normal or ulcerative colitis rats were assessed by sodium dodecyl sulphate polyacrylamide gel electrophoresis and were probed with Toll-like receptor (TLR) 9 as well as nuclear factor (NF)-κB p65 antibody. Antibody against β-actin was used as a loading control.