Effect of GP73 silencing on proliferation and apoptosis in hepatocellular cancer

Figure 6 Effects of silencing GP73 gene on apoptosis-related molecules in Hep G2 cells. A: Western blotting detected expression of GP73, Bcl-2, Bax, cytochrome c and procaspase-3 in Hep G2 cells at 48 h after transfection with Stealth RNAi (HSS181966; Stealth RNAi against GP73, negative control RNAi) or blank control; B-F: Representative immunoblots are shown from three independent experiments. Software Glyko Bandscan 5.0 was used to quantify the bands, and the expression of β-actin was used as a loading control, the protein levels were normalized against β-actin, data were expressed as mean ± SD (^bP < 0.01 vs blank group).