Effect of GP73 silencing on proliferation and apoptosis in hepatocellular cancer

Figure 1 BLOCK-iT Alexa Fluor red fluorescent control. The BLOCK-iT Alexa Fluor red fluorescent control (30 nmol/L) was transfected into Hep G2 and Bel7402 cells using Lipofectamine RNAi MAX Transfection Reagent. Twenty-four hours after transfection, growth medium was removed and replaced with PBS. Hep G2 and Bel7402 cell localization of the Alexa Fluor Red Fluorescent control oligo is seen with fluorescent microscopy (B and D, × 200). Over 80% of the cells took up the control oligo and retained a normal morphology, as seen in bright field (A and C, × 200).