Complex role for the immune system in initiation and progression of pancreatic cancer

Figure 2 Induction of innate immune response in a mouse model of acute pancreatitis. Mice were injected intraperitoneally with 50 μg/kg caerulein or saline hourly for 7 h (Mayo Clinic IACUC protocol A48510). The pancreas was isolated one hour following the last injection and analyzed immunohistochemically for the presence of neutrophils (Ly6B.2: AbSerotec) or macrophages (Mac-2; Cedarlane Diagnostics). A: Neutrophil infiltration in saline-treated mice; B: Neutrophil infiltration in caerulein-treated mice; C: Macrophage infiltration in saline-treated mice; D: Macrophage infiltration in cearulein-treated mice; E: Stained slides were imaged using ScanScope XT (Aperio, Vista, California) and immunohistochemical staining was quantified using the Aperio ImageScope reader. Mean ± SD is plotted for each group of n≥ 4 mice, ^bP < 0.01 vs saline group.