Role of the 13C-methacetin breath test in the assessment of acute liver injury in a rat model

Table 1 Comparison of parameters of the ¹³C-methacetin breath test and liver necrosis score between the control and model groups

Group	Tmax (min)	MVmax	CUM120	DOBmax	Liver necrosis score
Control	18.3 ± 4.1	5.06% ± 0.78%	4.21% ± 1.44%	75.1% ± 9.57%	0.4 ± 0.2
M6h	21.7 ± 4.7	3.84% ± 0.84%b	3.35% ± 0.72%a	52.3% ± 20.58%a	28.6 ± 18.5b
M12h	23.3 ± 5.2a	3.52% ± 1.32%b	2.77% ± 1.40%b	40.1% ± 4.13%b	59.5 ± 9.5b
M24h	33.3 ± 4.3b	2.55% ± 1.31%b	2.67% ± 0.71%b	37.3% ± 18.50%b	80.3 ± 4.2b
M48h	28.3 ± 3.8a	2.72% ± 0.31%b	2.77% ± 0.81%b	52.23% ± 14.16%a	36.0 ± 3.4b
M72h	23.3 ± 5.2a	4.72% ± 0.81%	4.12% ± 0.44%	69.1% ± 13.96%	21.1 ± 4.1a

DOB_max is the peak value of the delta over baseline (DOB) curve, which reflects the change in the natural ¹³CO₂/¹²CO₂ ratio exhaled to the ingestion of a labeled substrate during the test period. T_max reflects the time at which the peak amplitude of DOB curve appears. MV_max reflects the largest metabolic rate of ¹³CO₂, and CUM₁₂₀ reflects the cumulative percentage of administered dose of ¹³CO₂ recovered over time at 120 min.

^aP < 0.05,

^bP < 0.01 vs control.