Human liver stem/progenitor cells decrease serum bilirubin in hyperbilirubinemic Gunn rat

Figure 1 Adult-derived human liver stem/progenitor cells characterization. A: Morphology of adult-derived human liver stem/progenitor cells (ADHLSC) in expansion medium. Phase contrast microscopic view of ADHLSC obtained after second passage. Magnification: × 100; B: Immunocytochemistry characterization (passage 6) analysis: expression of mesenchymal and hepatic markers in ADHLSC. Mesenchymal markers: α-smooth muscle actin (αSMA) and vimentin and hepatic marker albumin immunostaining were analyzed by direct light microscopy. ADHLSC plated on collagen type I-coated coverslips were fixed and incubated with corresponding primary antibodies. Immunoreactivity was visualized using horseradish peroxydase (HRP) Cell nuclei are counterstaining with Mayer’s hematoxylin (blue). Images are representative of several fields examined.