Bortezomib effect on E2F and cyclin family members in human hepatocellular carcinoma cell lines

Figure 2 Microarray and quantitative real-time reverse transcription polymerase chain reaction assays in HepG2 following bortezomib treatment. A: The mRNA levels of the indicated E2Fs were evaluated by microarray analysis two days after JHH6 treatment with 40 nmol/L bortezomib. Data are reported as ratio between treated cells (T) and non-treated cells (NT); data are shown as means ± SEM ^aP < 0.05 vs controls; depending on the E2F member the number of evaluation ranged from three up to ten. B: The mRNA levels of the indicated E2Fs were evaluated by quantitative real-time reverse transcription polymerase chain assay; data, normalized to GAPDH mRNA, are shown as ratio between T and NT; data are reported as means ± SEM; ^aP < 0.05 vs controls, n = 5. RT-PCR: Reverse transcription polymerase chain reaction.