RAD51 potentiates synergistic effects of chemotherapy with PCI-24781 and cis-diamminedichloroplatinum on gastric cancer

Figure 4 RAD51 mediates the effect of PCI-24781. A, B: siRNA targeting RAD51 dramatically decreased the expression of RAD51; C: Depletion of RAD51 significantly increased the apoptotic cells ratio both in the PCI-24781- and cis-diamminedichloroplatinum (CDDP)-treated cells. A synergistic effect was observed in the combination treatment; D, E: For the overexpression system, human RAD51 cDNA was cloned into pcDNA3.1 to yield the pcDNA-RAD51 plasmid. The empty vector served as a control. The cells transfected with pcDNA-RAD51 showed a notable increase in RAD51 expression; F: Overexpression of RAD51 significantly decreased the apoptotic cells ratio both in the PCI-24781- and CDDP-treated cells. A synergistic effect was observed in the combination treatment. SiC: siRNA-control; SiR: siRNA-RAD51; Combo: Combination treatment. ^bP < 0.01 vs the control; ^cP < 0.05 vs PCI-24781 or CDDP; ^eP < 0.05 vs SiC or vector.