RAD51 potentiates synergistic effects of chemotherapy with PCI-24781 and cis-diamminedichloroplatinum on gastric cancer

Figure 2 PCI-24781 synergizes with the cis-diamminedichloroplatinum-induced gastric cancer cell apoptosis and clonogenic inhibition. A, B: After appropriate treatment [PCI-24781, cis-diamminedichloroplatinum (CDDP) and combination] for 48 h, the apoptosis of the HGC27 cells was measured using flow cytometry of annexin V labeled cells (A) and the quantum dot analysis of cleaved caspase-3 (B). The number of apoptotic cells was remarkably increased in the PCI-24781- or CDDP-treated groups, with a synergistic effect in the combination treated group; C, D: PCI-24781 or CDDP partially abrogates the colony formation capacity of the GC cells (10 d). The combination treatment exhibited a synergistic effect. Combo: Combination treatment. ^aP < 0.05 vs the control; ^cP < 0.05 vs PCI-24781 or CDDP.