KISS1 methylation and expression as predictors of disease progression in colorectal cancer patients

Figure 5 Changes in KISS1 expression after treatment of HCT116 cells with 5-Aza-CdR in vitro. A: KISS1 transcript expression in HCT116 cells was examined by real-time polymerase chain reaction after 5-Aza-CdR treatment for the indicated times. KISS1 expression was normalized to that of β-actin. The ratio of KISS1 expression in treated cells to that in untreated control cells is plotted on the Y-axis. KISS1 transcript expression gradually increased over time; B: Metastin expression levels after treatment with 5 μmol/L 5-Aza-CdR for different times were determined by Western blot. The same membranes were subsequently probed with an anti-β-actin antibody as a loading control; C: Metastin expression levels gradually increased over time; D: KISS1 transcript expression gradually increased with increasing concentrations of 5-Aza-CdR after 5 d; E: Metastin protein levels were determined by Western blotting after a 5-d treatment with different concentrations of 5-Aza-CdR; F: Metastin expression gradually increased with increasing concentrations of 5-Aza-CdR. The data are presented as the mean ± SE. ^aP < 0.05 vs untreated control.