KISS1 methylation and expression as predictors of disease progression in colorectal cancer patients

Figure 4 Changes in KISS1 methylation after treatment of HCT116 cells with different doses of 5-Aza-CdR. MS-polymerase chain reaction (PCR) for KISS1 in HCT116 cells after 5-Aza-CdR treatment. HCT116 cells were treated for 1 d, 3 d or 5 d with 0 μmol/L (control), 0.1, 1, 5 or 10 μmol/L 5-Aza-CdR. A PCR band in lane M indicates a methylated KISS1 gene; a band in lane U indicates an unmethylated KISS1 gene. Marker: 100-bp DNA ladder I; M: Methylated primer product; U: Unmethylated primer product.