KISS1 methylation and expression as predictors of disease progression in colorectal cancer patients

Figure 3 KISS1 methylation and KISS1 expression in various human colorectal cancer cell lines. A: MS-PCR for KISS1 in human colorectal cancer (CRC) cell lines. A PCR band in lane M indicates a methylated KISS1 gene, whereas a band in lane U indicates an unmethylated KISS1 gene; B: KISS1 gene expression in CRC cell lines was examined by real-time PCR, and the expression of KISS1 was normalized to that of β-actin. The ratio of KISS1 expression in other CRC cells to KISS1 expression in HCT116 cells is shown on the Y-axis; C: Metastin expression in CRC cells of the indicated genotypes was determined by Western blot. The same membranes were subsequently probed with an anti-β-actin antibody as a loading control; D: The metastin expression levels are presented as the mean ± SEM. ^aP < 0.05 vs HCT116 cells.