Original Article
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Aug 7, 2014; 20(29): 10071-10081
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
Figure 1
Figure 1 KISS1 methylation analysis of colorectal cancer tumor samples by methylation-specific polymerase chain reaction. Positive control: Placental tissue DNA treated with M.SssI methyltransferase; Negative control: DNA from normal lymphocytes. Tumors A and B were scored as positive/methylated due to the presence of a methylation band, whereas normal samples A and B were scored as negative/unmethylated due to the absence of a methylation band. The methylated polymerase chain reaction (PCR) product is 172 bp, and the unmethylated PCR product is 173 bp.