Aspirin inhibits cell viability and mTOR downstream signaling in gastroenteropancreatic and bronchopulmonary neuroendocrine tumor cells

Figure 5 The effect of aspirin on AKT/mTOR signaling in neuroendocrine BON1, NCI-H727 and GOT1 tumor cells. Human pancreatic neuroendocrine BON1 (A), human bronchopulmonary NCI-H727 (B) and midgut GOT1 (C) cells were treated with the indicated concentrations of aspirin for 24 and 48 h. The expression of pAkt, Akt, pTSC2, TSC2, pmTOR, mTOR and a β-actin loading control was subsequently evaluated using Western blot analysis. A representative blot from 3 independently performed experiments is shown.