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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Aug 7, 2014; 20(29): 10071-10081
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.10071
Table 2 Methylation-specific polymerase chain reaction primers and real-time polymerase chain reaction primers for KISS1
| Primer name | Sequence | Annealing temp (°C) | Product (bp) |
| KISS1 MSP primer | 5’-AAAGTTTCGTTTCGGAGGGTTC-3’ | 58 | 172 |
| 5’-CTTTTATAAAACCCGAAATAACG-3’ | |||
| KISS1 un-MSP primers | 5’-AAAGTTTTGTTTTGGAGGGTTT-3’ | 58 | 173 |
| 5’-AAAGTTTTGTTTTGGAGGGTTT-3’ | |||
| KISS1 real-time PCR primers | 5’-ACCTGGCTCTTCTCACCAAG-3’ | 60 | 201 |
| 5’-TAGCAGCTGGCTTCCTCTC-3’ | |||
| β-actin primers | 5’-ATCTGGCACCACACCTTCTACAATGAGCTGCG-3’ | 60 | 837 |
| 5’-CGTCATACTCCTGCTTGCTGATCCACATCTGC-3’ |
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Citation: Chen SQ, Chen ZH, Lin SY, Dai QB, Fu LX, Chen RQ.
KISS1 methylation and expression as predictors of disease progression in colorectal cancer patients. World J Gastroenterol 2014; 20(29): 10071-10081 - URL: https://www.wjgnet.com/1007-9327/full/v20/i29/10071.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i29.10071