Establishment of an orthotopic pancreatic cancer mouse model: Cells suspended and injected in Matrigel

Figure 3 Tumor formation and intra-abdominal tumor implantation. Pan02 cells were suspended in low-temperature phosphate buffered saline (PBS) or Matrigel, and injected into the pancreas tail of C57BL/6 mice. Half of the mice were sacrificed at 2 wk, with the remainder being sacrificed at 3 wk after injection. Tumor formation (white arrow) at the injection site and intra-abdominal tumor disseminated foci (white arrowhead) were thoroughly examined and marked.