Mast cell deficiency exacerbates inflammatory bowel symptoms in interleukin-10-deficient mice

Table 1 Primer sets used for quantitative reverse transcriptase-polymerase chain reaction analysis of mouse colonic tissue

Gene name	Accession No.	Product size	Direction	Sequence (5’ to 3’)	Ref.
Claudin2	NM_016675.4	120 bp	Forward	GGCGTCCAACTGGTGGGCTAC	[42]
			Reverse	AACCGCCGTCACAATGCTGGC
Claudin3	NM_009902.4	132 bp	Forward	CAGGGGCAGTCTCTGTGCGAG	[42]
			Reverse	GCCGCTGGACCTGGGAATCAAC
GAPDH	NM_008084.2	132 bp	Forward	AACTTTGGCATTGTGGAAGG	[42]
			Reverse	GGATGCAGGGATGATGTTCT
IL-1β	NM_008361	73 bp	Forward	TCGCTCAGGGTCACAAGAAA	[43]
			Reverse	CATCAGAGGCAAGGAGGAAAAC
INFγ	NM_008337.3	93 bp	Forward	AGGTCCAGCGCCAAGCATTCAA	[42]
			Reverse	AGCAGCGACTCCTTTTCCGCTT
iNOS	U43428.1	76 bp	Forward	CAAAGTCTCAGACATGGCTTGC	This study
			Reverse	TTCCTCTGTCAGGTCACTTTGG
NOX1	NM_172203.1	113 bp	Forward	CAGGCATCCTCATTTTGCGG	This study
			Reverse	CCTTCTGCTGGGAGCGATAA
T-bet	NM_019507.2	138 bp	Forward	CCACTGGATGCGCCAGGAAGTT	[42]
			Reverse	TTCACCTCCACGATGTGCAGCC
TNF-α	NM_013693.2	67 bp	Forward	TGGGACAGTGACCTGGACTGT	[43]
			Reverse	TTCGGAAAGCCCATTTGAGT