Clobenpropit enhances anti-tumor effect of gemcitabine in pancreatic cancer

Figure 1 Western blotting of histamine receptors in pancreatic cancer cells. Cellular extracts of Panc-1, MiaPaCa-2 and AsPC-1 were separated by SDS-PAGE, transblotted onto nitrocellulose membranes and detected using anti-H₄ receptor antibodies. Pancreatic cancer cell lines were treated with different concentrations of clobenpropit and/or gemcitabine, and Western blotting was performed. The specific antibody to H₄ receptor showed immunoreactivity mainly as a band at 70000 and 43000 Da. Expression of H₄ receptor was still present after administration of clobenpropit alone (A) or combination with gemcitabine (B).