High fat diet feeding results in gender specific steatohepatitis and inflammasome activation

Figure 6 Long-term high fat diet induced inflammasome upregulation and activation in male mice. Hepatic mRNA expression of the inflammasome components NOD-like receptors3 (NLRP3), caspase-1, adaptor molecule, Aim2, and NLRC4 were measured using real-time quantitative polymerase chain reaction in male (A) and female (B) mice after a long-term high fat diet. The functional activity of the inflammasome was evaluated by measurements of caspase-1 activity (C) and total nterleukin (IL)-1β protein (D) levels, in the liver. ^aP < 0.05, ^bP < 0.01 vs chow fed controls, n = 10/group.