APE1 polymorphisms are associated with colorectal cancer susceptibility in Chinese Hans

Table 1 Primer sequences

Target gene	Sequence of primers	Allele and PCR product size (bp)
OGG1	F1: 5’-CAGCCCAGACCCAGTGGACTC-3’	C allele (252 bp)
Ser326Cys	R1: 5’-TGGCTCCTGAGCATGGCGGG-3’
	F2: 5’-CAGTGCCGACCTGCGCCAATG-3’	G allele (194 bp)
	R2: 5’-GGTAGTCACAGGGAGGCCCC-3’
XRCC1	F1: 5’-TCCCTGCGCCGCTGCAGTTTCT-3’	G allele (447 bp)
Arg399Gln	R1: 5’-TGGCGTGTGAGGCCTTACCTCC-3’
	F2: 5’-TCGGCGGCTGCCCTCCCA-3’	A allele (222 bp)
	R2: 5’-AGCCCTCTGTGACCTCCCAGGC-3’
APE1	F1: 5’-CCTACGGCATAGGTGAGACC-3’	G allele (167 bp)
Asp148Glu	R1: 5’-TCCTGATCATGCTCCTCC-3’
	F2: 5’-TCTGTTTCATTTCTATAGGCGAT-3’	T allele (236 bp)
	R2: 5’-GTCAATTTCTTCATGTGCCA-3’
APE1 promoter	F1: 5’-CTAACTGCCAGGGACGCCGA-3’	T allele (136 bp)
-141T/G	R1: 5’-ACACTGACTTAAGATTCTAACTA-3’
	F2: 5’-ACTGTTTTTTTCCCTCTTGCACAG-3’	G allele (335 bp)
	R2: 5’-TGAGCAAAAGAGCAACCCCG-3’

PCR: Polymerase chain reaction; OGG1: 8-oxoguanine DNA-glycosylase 1; APE1: Apurinic endonuclease 1; XRCC1: X-ray repair cross-complementing protein 1.