Changes in circulating Foxp3+ regulatory T cells and interleukin-17-producing T helper cells during HBV-related acute-on-chronic liver failure

Table 2 T helper type 17 and T regulatory differentiation-related cytokines

Genes	GenBank accession number	Primers (5' to 3')	Reported function
IL-1β	NM_000576	Forward: GCTGATGGCCCTAAACAGATGAA	Induce human Th17 polarization[17]
		Reverse: TGAAGCCCTTGCTGTAGTGGTG
IL-6	NM_000600	Forward: AAGCCAGAGCTGTGCAGATGAGTA	Induce human Th17 polarization[17]
		Reverse: TGTCCTGCAGCCACTGGTTC
IL-23/p19	NM_016584	Forward: GCAGCCTGAGGGTCACCACT	Unique subunit of IL-23
		Reverse: GGCGGCTACAGCCACAAA
IL-17A	NM_002190	Forward: TGTCCACCATGTGGCCTAAGAG	Main effective cytokine of Th17 cells[7]
		Reverse: GTCCGAAATGAGGCTGTCTTTGA
TGF-β1	NM_000660	Forward: AGCGACTCGCCAGAGTGGTTA	Induce mouse Th17 and Treg polarization, suppress human Th17 polarization[9,10,17]
		Reverse: GCAGTGTGTTATCCCTGCTGTCA
IL-2	NM_000586	Forward: CAACTCCTGTCTTGCATTGCACTAA	Induce human and mouse Treg polarization[10]
		Reverse: AATGTGAGCATCCTGGTGAGTTTG
FoxP3	NM_014009	Forward: GTTCACACGCATGTTTGCCTTC	Master regulatory transcription factors of Treg lineage[21]
		Reverse: CACAAAGCACTTGTGCAGACTCAG