Effect of a high-fat diet in development of colonic adenoma in an animal model

Figure 3 Representative photomicrographs showing the histological characteristics and proliferative state of colonic adenoma. A and B: Histological characteristics of colonic adenoma in the normal-fat diet/high-fat diet (ND/HFD) groups, and the magnified micrograph (right) highlights the area from the corresponding white rectangle (left); C and D: Representative immunohistochemical staining of proliferative activity (Ki-67, brown) of colonic epithelial cells in the ND/HFD groups; C: Adjacent normal epithelial cells; D; Colonic adenoma tissue; E and F: Ki-67-positive cells were quantified for adjacent normal epithelium (E) and adenoma tissue (F) by counting the positive cells per field (n = 5 per group, ^bP < 0.01, HFD-fed vs ND-fed). The magnification is marked in the lower right of the pictures, respectively. MM: Magnified micrograph.