Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas

Figure 2 Mesothelin knockdown in INS-1 cells. Real-time polymerase chain reaction (PCR) assay (A) and western blot assay (B) of mesothelin in INS-1 cells transfected with pcDNA6.2-GW/EmGFP-miR, pcDNA6.2-GW/EmGFP-miR-mesothelin (301), or pcDNA6.2-GW/EmGFP-miR-mesothelin (1275). The qPCR data are normalized against GAPDH; C: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were conducted to determine the proliferation of INS-1 cells; D: Bar chart represents the percentage of cells in G0-G1, S, or G2-M phase, as indicated; E: Colony-forming growth assays were conducted to determine the proliferation of INS-1 cells and were quantified by spectrophotometer at O.D.450; F: Apoptotic rates of cells were detected by flow cytometry. Values are expressed as the mean ± SD from three experiments. ^aP < 0.05 vs mock group. UL: Necrotic cells; UR: Terminal apoptotic cells; LR: Early apoptotic cells.