Multispecies probiotic protects gut barrier function in experimental models

Figure 1 Effect of the Lactibiane Tolerance^®. Strain combination on T84 monolayer permeability in basal conditions (A) and after stimulation with 10 ng/mL (B) or 100 ng/mL lipopolysaccharide (C). Experiments were performed in duplicate and data were normalized by comparison to control wells containing culture medium only. The results displayed on the left panels are the means of 5 experiments in basal conditions and 3 experiments with lipopolysaccharide (LPS) stimulation. The right panels show the dispersion of values and statistical significance of the differences evaluated with the one-way Anova analysis followed by the Bonferroni post-test set at P < 0.05 and is displayed as ^aP < 0.05, ^bP < 0.01 or ^dP < 0.01 vs culture medium + LPS; D: On ZO-1 and occludin expression in basal conditions (a) or in the presence of 10 ng/mL LPS (b). Expression of occludin (65 kDa) and ZO-1 (220 kDa) was evaluated by immunoblotting. Calnexin (95 kDa) was used as a loading control.