Carcinogenic Helicobacter pylori in gastric pre-cancer and cancer lesions: Association with tobacco-chewing

Table 2 Primer sequences, primer annealing positions, expected lengths of amplified DNA products, and amplification cycle of polymerase chain reaction performed in the study

Primer	Sequence	Annealing position	Product length (bp)	Amplification cycle
Hp ureC-F	5’-AAG CTT TTA GGG GTG TTA GGG GTT T-3’	1293-1318	294	94 °C, 10’, 36 cycle (94 °C, 2’; 55 °C, 2’; 72 °C, 2’), 72 °C, 10’
Hp ureC-R	5’-AAG CTT ACT TTC TAA CAC TAA CGC-3’	1587-1563
Hp cagA-F	5’-AAT ACA CCA ACG CCT CCA AG-3’	2593-2612	400	94 °C, 4’, 39 cycle (94 °C, 2’; 59 °C, 2’; 72 °C, 2’), 72 °C, 10’
Hp cagA-R	5’-TTG TTG CCG CTT TTG CTC TC-3’	2992-2973
β-globin-F	5’-GAA GAG CCA AGG ACA GGT AC-3’	195-176	268	94 °C, 4’, 30 cycle (95 °C, 30’’; 55 °C, 30’’; 72 °C, 1’), 72 °C, 10’
β-globin-R	5’-CAA CTT CAT CCA CGT TCA ACC-3’	54-73