Protocol liver biopsy is the only examination that can detect mid-term graft fibrosis after pediatric liver transplantation

Table 4 Clinical and hitopathological findings of cases with ≥ A2 or ≥ F2 of protocol liver biopsy at two or five years after living donor liver transplantation

Case	Original disease	Age at PLB/sex	Previous ACR	Post-transplant complications	IS at PLB	Tac trough at PLB	ALT/HA at PLB	A/F at PLB	IS at follow-up biopsy	A/F at follow-up biopsy
PLB at two years after LDLT
1	OTCD	71/female	-	-	Tac (3.0)	2.5	12/35	2/1	Tac (1.0)/MMF (400)	1/1
2	OTCD	164/female	-	BDS	Tac (2.0)/MMF (1000)	5.2	34/13	2/1	Tac (2.0)/MMF (1000)	1/0
3	OTCD	44/male	-	-	Tac (0.8)/MMF (250)	2	25/< 9	2/2	Tac (0.8)/MMF (500)	N.E.
PLB at five years after LDLT
4	BA	70/female	+	Bowel perforation	Tac (0.6)	1.1	22/13	2/2	Tac (2.0)/MMF (1000)	0/0
5	BA	118/female	-	-	Tac (1.0)	2.3	20/24	2/2	Tac (2.0)/MMF (1000)	1/0
6	BA	70/female	+	HAT/IHBDS	Tac (0.8)/MMF(500)	3.6	32/28	1/2	Tac (2.0)/MMF (500)	1/1
7	BA	71/female	-	CMV-I	Tac (0.4)	0	16/< 9	2/2	Tac (1.6)	N.E.
8	FHF	83/female	-	-	Tac (2.0)/MMF (500)	2.2	26/< 9	1/2	Tac (2.8)/MMF (500)	N.E.
9	BA	77/female	-	CMV-I	Tac (0.4)	2.6	14/29	2/3	Tac (0.8)	0/1
10	BA	84/female	+	Fungal infection	Tac (0.4)	2.1	26/11	2/2	Tac (0.4),MMF (500)	1/1
11	BA	89/male	+	PVS	Tac (1.6)/MP (4.0)/MMF (1500)	2.2	12/17	2/2	Tac (1.6)/MP (2.0)/MMF (1500)	1/1
12	BA	174/male	-	BDS	Tac (3.0)	2.3	16/20	1/2	Tac (4.0)	0/1
13	BA	69/female	+	CMV-I	Tac (1.6)	2.8	18/< 9	1/2	Tac (2.0)/MMF (1000)	0/1
14	BA	84/male	-	HVS	Tac (2.0)/MP (1.0)/MMF (1000)	5.6	12/23	1/2	Tac (2.0)/MP (1.0)/MMF (1000)	N.E.

PLB: Protocol liver biopsy; LDLT: Living donor liver transplantation; ACR: Acute cellular rejection; IS: Immunosuppressants; Tac: Tacrolimus; ALT: Alanine amino transferase; HA: Hyaluronic acid; A: Activity; F: Fibrosis; OTCD: Ornithine transcarbamylase deficiency; BA: Biliary atresia; FHF: Fulminant hepatic failure; BDS: Biliary duct anastomotic stenosis; HAT: Hepatic artery thrombosis; IHBDS: Intrahepatic biliary duct stenosis; CMV-I: Cytomegalovirus infection; PVS: Portal vein stenosis; HVS: Hepatic vein stenosis; MMF: Mycophenolate mofetil: MP: Methylprednisolone.