Protocol liver biopsy is the only examination that can detect mid-term graft fibrosis after pediatric liver transplantation

Table 3 Risk factors for ≥ A1 and ≥ F1 of protocol liver biopsy at two and five years after living donor liver transplantation: multivariate analysis

Variables	OR	95%CI	P value
Risk factors for ≥ A1 of PLB at two years after LDLT
HLA-A mismatch
Mismatch vs match	0.46	0.145-1.479	0.194
Cold ischemic time
≥ 2 h vs < 2 h	4.15	1.164-14.789	0.028
Acute cellular rejection
Yes vs No	0.20	0.046-0.878	0.033
ALT
≥ 20 IU/L vs < 20 IU/L	4.64	1.409-15.306	0.012
Hyaluronic acid
≥ 20 ng/mL vs < 20 ng/mL	3.30	0.982-11.076	0.054
Risk factors for ≥ F1 of PLB at two years after LDLT
Recipient age
< 1 yr vs≥ 1 yr	1.54	0.506-4.706	0.446
AST
≥ 30 IU/L vs < 30 IU/L	2.68	0.775-9.238	0.120
ALT
≥ 20 IU/L vs < 20 IU/L	1.86	0.646-5.335	0.251
Risk factors for ≥A1 of PLB at five years after LDLT
Cold ischemic time
≥ 2 h vs < 2 h	2.94	0.778-11.140	0.112
Acute cellular rejection
Yes vs No	2.26	0.728-7.035	0.158
Risk factor for ≥ F1 of PLB at five years after LDLT
Acute cellular rejection
Yes vs No	2.75	0.876-8.637	0.083

PLB: Protocol liver biopsy; LDLT: Living donor liver transplantation; ALT: Alanine amino transferase; AST: Aspartate amino transferase.