Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation

Table 1 List of primers used for PCR assays

Primer name	Sequence (5’-3’)	Region specific for	Ref.
afa-f	CGGCTTTTCTGCTGAACTGGCAGGC	afaC	[49]
afa-r	CCGTCAGCCCCCACGGCAGACC
afa1	GCTGGGCAGCAAACTGATAACTCTC	afaBC
afa2	CATCAAGCTGTTTGTTCGTCCGCCG
afaE-f1	TTAGACCGTACTGTTGTGTTACCCCC
afaE1-r	CATCGCCCGTCGCAGAGCCCAT	afaE1
afaE2-r	GTTTCCCAGTAGACTGGAATGAAGC	afaE2
afaE3-r	CCCTATTGTTGTCGCTGATCAGGAAG	afaE3
daaE-r	CGGCTAGTCATATATAGATTTGTCGC	daaE
afaE5-f	TCAACTCACCCAGTAGCCCCAG	afaE5
afaE5-r	AGGAAGTGGTAGCACCGGTACG
afaE7-f	GCTAAATCAACTGTTGATGTT	afaE7
afaE7-r	GGACAATCCAAATGGCGAATTA
afaE8-f	CTAACTTGCCATGCTGTGACAGTA	afaE8
afaE8-r	TTATCCCCTGCGTAGTTGTGAATC
aggR1	CTAATTGTACAATCGATGTA	aggR	[50]
aggR2	CTGAAGTAATTCTTGAA
pksORF9-10.1KJ	ATTCGATAGCGTCACCCAAC	clbK-clbJ	[51]
pksORF9-10.2KJ	TAAGCGTCTGGAATGCAGTG
CNF-1s	GGGGGAAGTACAGAAGAATTA	cnf1	[51]
CNF-1as	TTGCCGTCCACTCTCACCAGT
CNF-2s	TATCATACGGCAGGAGGAAGCACC	cnf2
CNF-2as	GTCACAATAGACAATAATTTTCCG
CNF3-3D	TAACGTAATTAGCAAAGA	cnf3
CNF-3as	GTCTTCATTACTTACAGT
CDT-s1	GAAAGTAAATGGAATATAAATGTCCG	cdtB-II, cdtB-III, cdtB-V	[51]
CDT-as1	AAATCACCAAGAATCATCCAGTTA
CDT-IIas2	TTTGTGTTGCCGCCGCTGGTGAAA	cdtB-II
CDT-IIIas2	TTTGTGTCGGTGCAGCAGGGAAAA
CDT-s2	GAAAATAAATGGAACACACATGTCCG	cdtB-I, cdtB-IV
CDT-as2	AAATCTCCTGCAATCATCCAGTTA
CDT-Is	CAATAGTCGCCCACAGGA	cdtB-I
CDT-Ias	ATAATCAAGAACACCACCAC
CDT-IVs	CCTGATGGTTCAGGAGGCTGGTTC	cdtB-IV
CDT-IVas	TTGCTCCAGAATCTATACCT
P105	GTCAACGAACATTAGATTAT	cdtC-V
c2767r	ATGGTCATGCTTTGTTATAT
cif-int-s	AACAGATGGCAACAGACTGG	cif	[51]
cif-int-as	AGTCAATGCTTTATGCGTCAT
clbQ-F	TTGTATAGTTACACAACTATTTC
clbQ-R	CCTGTTAGCTTTCGTTC		This study
MIclbQaadA7-F	CATTAAATCATCAAATTAAACGAATTCTATTACACAACAAGGAGTGGGACGCACTGGCATTTAATAACGCGTC
MIClbQaadA7-R	GATGATGGAACAGCCATATCTATTGCTCCTTGTATAGTTACACAACTATTTTTAATCACTTTACTTTTATC

¹The afa-f and afa-r primers detect all afa-positive strains, irrespective of the afaE subtype and the binding properties of the adhesins (Afa/Dr+ and Afa/Dr-). Specific detection of strains encoding Afa/Dr+ adhesins was performed using afa-1 and afa-2 primers;

²Primers used with CDT-s1 primer.