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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. May 28, 2014; 20(20): 6329-6335
Published online May 28, 2014. doi: 10.3748/wjg.v20.i20.6329
Published online May 28, 2014. doi: 10.3748/wjg.v20.i20.6329
Table 2 SFRP2 and WIF-1 gene primer sequences for methylation-specific polymerase chain reaction assays
| Gene | Primer (5’-3’) | Product size (bp) | Annealing temperature (°C) |
| M: SFRP2 | GGGTCGGAGTTTTTCGGAGTTGCGC | 138 | 62 |
| CCGCTCTCTTCGCTAAATACGACTCG | (38) | ||
| U: SFRP2 | TTTTGGGTTGGAGTTTTTTGGAGTTGTGT | 145 | 52 |
| AACCCACTCTCTTCACTAAATACAACTCA | (38) | ||
| M: WIF-1 | GGGCGTTTTATTGGGCGTAT | 197 | 58 |
| AAACCAACAATCAACGAAC | (38) | ||
| U: WIF-1 | GGGTGTTTTATTGGGTGTAT | 198 | 55 |
| AAACCAACAATCAACAAAAC | (38) |
- Citation: Zhang H, Zhu YQ, Wu YQ, Zhang P, Qi J. Detection of promoter hypermethylation of Wnt antagonist genes in fecal samples for diagnosis of early colorectal cancer. World J Gastroenterol 2014; 20(20): 6329-6335
- URL: https://www.wjgnet.com/1007-9327/full/v20/i20/6329.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i20.6329