Deletion of Gpr128 results in weight loss and increased intestinal contraction frequency

Figure 2 Selective expression of Gpr128 within the intestine in mice. A: Expression levels of Gpr128 mRNA. The mRNA levels were examined in major tissues of normal mice using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression level of β-actin was used as an endogenous control. M: Marker lane; (-): Negative control without template; B: Northern blotting analysis of Gpr128. Total RNA from wild type mice was extracted and subjected to Northern blotting analysis using a 715-bp fragment of Gpr128 cDNA corresponding to exons 1 through 6. The bottom lane shows the 28S and 18S ribosomal RNA as a control; C: Examination of the stage-specific expression of Gpr128 mRNA. RT-PCR was performed throughout the digestive tract and at various postnatal developmental stages to determine the presence of Gpr128 mRNA from postnatal day 0 through 8 wk; D: Expression pattern of Gpr128 protein in the stomach and colon of adult WT mouse revealed by immunofluorescence (original magnification, × 200).