Proteomic identification of tumor biomarkers associated with primary gallbladder cancer

Figure 2 Western blot analysis of proteins. Expression of ANXA4, Hsp90β, Dync1h1, ACTG, ACTA2 and α-vinculin in paired gallbladder cancer tissues (Ca) and normal tumor-adjacent tissues (N). Quantification was performed by densitometry of Western blot bands. Densities were normalized to α-vinculin, and relative folds were normalized to expression levels in normal tumor-adjacent tissues. The data are representative of 20 paired groups. ANXA4: Antibody against annexin A4; ACTG: Gamma-actin; ACTA2: Aortic smooth muscle actin; Dync1h1: Dynein cytoplasmic 1 heavy chain 1; Hsp90β: Heat shock protein 90-beta.