Use of lectin microarray to differentiate gastric cancer from gastric ulcer

Table 2 Differential glycopattern in gastric ulcer and cancer according to the lectin microarray analysis based on data from 37 lectins giving significant signals

Lectin	Specificity	Normalized fluorescent intensity1		Ratio, P value
		Gastric cancer	Gastric ulcer	(C/U)
Jacalin	Galβ1-3GalNAcα-Ser/Thr(T) and GalNACα-Ser/Thr(Tn)	0.49	-	P < 0.001
ECA	Galβ-1,4GlcNAc	0.72	-	P < 0.001
HHL	Polymannose (α-1,3) and (α-1,6) linked mannose	0.64	-	P < 0.001
WFA	GalNAcα/β-1,3/6Gal	0.64	-	P < 0.001
GSL-II	GlcNAc	0.67	-	P < 0.001
MAL-II	Sia2-3Galβ1-4Glc (NAc)	-	-	/
PHA-E	Bisecting GlcNAc and biantennary N-glycans	0.65	-	P < 0.001
PTL-I	αGalNAc	0.67	-	P < 0.001
SJA	Terminal in GalNAc and Gal	0.12	0.38	0.32, P < 0.001
PNA	Galβ1-3GalNAcα-Ser/Thr(T)	0.08	0.22	0.36, P < 0.001
EEL	Galα1-3(Fucα1-2)Gal	1	0.23	4.41, P < 0.001
AAL	Fucα-1,6GlcNAc, Fucα-1,3LacNAc	0.89	-	P < 0.001
LTL	α-L-Fuc	0.59	0.31	/
MPL	αGalNAc	0.69	-	P < 0.001
LEL	Poly-LacNAc and (GlcNAc)n	0.54	0.27	/
GSL-I	αGalNAc, αGal	-	0.03	P < 0.001
DBA	GalNAcα-Ser/Thr(Tn)	0.4	0.2	/
LCA	α-Man and Fucα-1,6GlcNAc (core fucose)	0.61	-	P < 0.001
RCA120	Gal and GalNAc	0.61	0.17	3.55, P < 0.001
STL	(GlcNAc)n	0.54	0.31	/
BS-I	αGal and αGalNAc	0.12	-	P < 0.001
ConA	α-Man (inhibited by presence of bisecting GlcNAc)	0.13	0.11	/
PTL-II	Gal	0.66	-	P < 0.001
DSA	β1-4GlcNAc and LacNAc	0.79	1	0.79, P < 0.001
SBA	Terminal GalNAc (especially GalNAcα1-3Gal)	0.38	-	P < 0.001
VVA	GalNAc and GalNAcα-Ser/Thr(Tn)	0.45	-	P < 0.001
NPL	Polymannose(α-1,6)linked mannose	0.64	0.12	5.21, P < 0.001
PSA	Fucα-N-acetylchitobiose -man	0.85	0.2	4.19, P < 0.001
ACA	Galβ1-3GalNAcα-Ser/Thr(Tn)	0.71	0.18	3.84, P < 0.001
WGA	Terminal GlcNAc and (GlcNAc)n	0.35	-	P < 0.001
UEA-I	Fucoseα1-2Galβ1-4Glc(NAc)	0.81	-	P < 0.001
PWM	GlcNAc	0.8	-	P < 0.001
MAL-I	Galβ-1,4GlcNAc and Sia2-3Galβ1-4Glc(NAc)	0.63	0.16	3.89, P < 0.001
GNA	(α-1,3)Man	0.78	0.09	9.1, P < 0.001
BPL	Galβ1-3GalNAc	0.67	-	P < 0.001
PHA-E+L	Tri-and tetra-antennary complex-type N-glycan	0.92	-	P < 0.001
SNA	Sia2-6Galβ1-4Glc(NAc)	0.61	0.18	3.39, P < 0.001

¹Signal intensities obtained from nine replicate blocks on three replicate slides were normalized and averaged, and the ratio of gastric cancer vs gastric ulcer (C/U) was calculated. -: Negative signal; /: No significant difference.