Naofen promotes TNF-α-mediated apoptosis of hepatocytes by activating caspase-3 in lipopolysaccharide-treated rats

Figure 3 Effects of exposure period of Kupffer cells to lipopolysaccharide on tumor necrosis factor-α production. Primary Kupffer cells (KCs) were separated from rat livers using collagenase perfusion. After 24 h of incubation, KCs were incubated with lipopolysaccharide (LPS) (100 ng/mL). A: Tumor necrosis factor (TNF)-α mRNA was quantified using qPCR (n = 6); B: Immunoblotting assay for TNF-α. KC lysates were analyzed with TNF-α antibody (n = 6). GAPDH was used as an internal control. ^aP < 0.05, ^bP < 0.01 and ^dP < 0.001 vs controls (without LPS treatment).