Naofen promotes TNF-α-mediated apoptosis of hepatocytes by activating caspase-3 in lipopolysaccharide-treated rats

Figure 2 Effects of anti-tumor necrosis factor-α antibody on caspase-3 activation and apoptosis. Rats were treated as described above. A: Caspase-3 activation. Livers were lysed in lysis buffer and caspase-3 activation was measured. ^bP < 0.01 vs controls; ^dP < 0.01 vs LPS + IgG (n = 10); B: Liver apoptosis was examined using dUTP nick end labeling assay in rats 9 h after injection with control (saline + IgG), LPS + IgG or LPS + anti-TNF-α. Bar: 100 μm; C: Apoptosis index in the different groups. ^bP < 0.01 vs control; ^dP < 0.01 vs LPS + IgG (n = 10). TNF-α: Tumor necrosis factor-α; LPS: Lipopolysaccharide.