Localization and vasopressin regulation of the Na+-K+-2Cl- cotransporter in the distal colonic epithelium

Figure 3 Western blotting analysis of total and plasma membrane Na⁺-K⁺-2Cl^- cotransporter protein after saline or 1-D-Amino(8-D-arginine)-vasopressin injection. A: Each lane was loaded with 20 μg of protein from the mouse distal colon and kidney homogenates (control and dDAVP-treated n = 3 mice); B: Each lane was loaded with 40 μg of protein from the plasma membrane of the colonic mucosa (n = 4 mice); C: Summary of the densitometric analysis of the membrane NKCC2, normalized to GAPDH. Densitometric analysis revealed that the expression of NKCC2 was significantly higher in the treated mice than in the control mice (^fP < 0.01; ^hP < 0.01, n = 3).