Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice

Figure 3 Bifico increased expression of tight junction proteins in interleukin-10 gene-deficient mice. A: Representative immunofluorescence photomicrographs for tight junction proteins zonula occludentes (ZO)-1, in the colonic epithelium (n = 3 for each group); B: Representative immunofluorescence photomicrographs for tight junction proteins claudin-1 (n = 3 for each group); C: Representative immunofluorescence photomicrographs for tight junction proteins occludin (n = 3 for each group).The tight junction proteins were stained red (× 400 magnification); D: Western blotting of tight junction proteins in the colonic tissues; E: mRNA expression of ZO-1 in the colon (mean ± SEM, n = 4-6 per group, ^aP < 0.05, Student’s t test); F: mRNA expression of claudin-1 in the colon (mean ± SEM, n = 4-6 per group, ^aP < 0.05, Student’s t test); G: mRNA expression of occludin in the colon (mean ± SEM, n = 4-6 per group, ^aP < 0.05, Student’s t test).