Squamous cell carcinoma after radiofrequency ablation for Barrett's dysplasia

Figure 1 Endoscopic, genetic and histological figures of the patient before and immediately after radiofrequency ablation for Barrett’s related high grade dysplasia. A: Sanger sequencing from columnar lined oesophagus taken in June 2010 (2) showing a mutation in CDKN2A c.286 G > A (p.V100M) (starred). A control sequence is also shown (1); B: Haematoxylin and eosin stain at × 10 magnification from a biopsy taken in June 2010. This demonstrates high grade dysplasia with nuclear pleiomorphism, increased nuclear: cytoplasmic ratio and disordered nuclei (arrow); C: Endoscopic view of the patient’s columnar-lined oesophagus (April 2009) showing classical salmon pink mucosa between 28cm and 36cm from the incisors; D: Endoscopic view of the patient’s re-epithelialized oesophagus (September 2010) after radiofrequency ablation.