Non-transmissible Sendai virus vector encoding c-myc suppressor FBP-interacting repressor for cancer therapy

Figure 3 High efficiency of Sendai virus/dF/green fluorescent protein vectors to HeLa cells and Sendai virus/dF/green fluorescent protein vectors indicates significant cell growth inhibition with apoptosis. A: HeLa cells were infected with a 10 MOI of SeV/dF/FIR virus vector and cultured in DMEM for 3 d (72 h). The same amount of 10 MOI SeV/dF/GFP was also used to infect the control. As shown in the left lower panel, SeV/dF/GFP infected almost 100% cells (green). Under these conditions, SeV/dF/FIR significantly inhibited cell growth as shown in the left upper panel. Mock and 10 MOI SeV/dF/GFP showed no cell growth inhibition as seen in the right upper and lower panels; B: HeLa cells infected by 10 MOI SeV/dF/FIR for 3 d showed significant cell damage revealed by the APO Percentage apoptosis assay™, compared to mock or the same conditions of SeV/dF/GFP virus infected HeLa cells. 5 mmol/L H₂O₂ was used as a positive control. FIR: FBP Interacting Repressor; FBP: FUSE-Binding protein; FUSE: Far Upstream Element; SeV: Sendai virus; GFP: Green fluorescent protein; MOI: Multiplicity of infection.