Regulatory effect and mechanisms of carbon monoxide-releasing molecule II on hepatic energy metabolism in septic mice

Figure 6 Effect of carbon monoxide-releasing molecule II on hepatic mitochondrial function in septic mice. Mice were challenged with cecal ligation and puncture (CLP) and treated with tricarbonyldichlororuthenium (II) dimer (CORM-2) or iCORM-2 as described in the Methods section. Isolated mitochondria were resuspended in the assay buffer. Ca²⁺-induced mitochondrial swelling was assayed by the decrease in absorbance at 540 nm. The CLP group of mice had significant hepatic mitochondrial swelling with severely damaged mitochondrial function. Following CORM-2 intervention, the extent of hepatic mitochondrial swelling was reduced and mitochondrial function was clearly protected (P < 0.05 vs CLP mice). The curves represent typical recordings from experiments of at least three different mitochondrial preparations.