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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 21, 2014; 20(11): 2979-2994
Published online Mar 21, 2014. doi: 10.3748/wjg.v20.i11.2979
Published online Mar 21, 2014. doi: 10.3748/wjg.v20.i11.2979
Figure 9 Transient transfection of KRAS siRNA in DLD-1 (KRAS) and Caco2 (Wt) cell line.
Expression of chemokine (C-X-C motif) ligand (CXCL)-1/Gro-alpha (A) and CXCL10/IP-10 (B) in the DLD-1 (KRAS-mutant) cell-line. Expression of CXCL-1/Gro-alpha (C) and CXCL-10/IP-10 (D) in the Caco2 (WT) cell-line. 5 × 104 cells were plated into 24 well plates and grown for 24 h and then transfected with KRAS siRNA or scrambled (20 nmol/L) for 72 h. Real time PCR was performed for chemokine CXCL1 and CXCL10 with gene specific primers and the expression was normalized to β-actin expression measured in the same sample as an internal control. Data presented are the mean ± SE of 4 independent experiments with double confirmation. aP < 0.05 vs scrambled 72 h.
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Citation: Khan S, Cameron S, Blaschke M, Moriconi F, Naz N, Amanzada A, Ramadori G, Malik IA. Differential gene expression of chemokines in
KRAS andBRAF mutated colorectal cell lines: Role of cytokines. World J Gastroenterol 2014; 20(11): 2979-2994 - URL: https://www.wjgnet.com/1007-9327/full/v20/i11/2979.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i11.2979